| Electron microscopy |
Preparation of fixatives |
| Processing and embedding of tissues or cultured cells in epoxy resins or acrylics |
| Preparation of tissues for scanning electron microscopy |
| Critical point drying HMDS drying Mounting Sputter coating |
| Sectioning |
| Thick (1-micron) sectioning with toluidine blue stain Ultrathin sectioning – plastic embedded material or frozen samples Vibratome |
| Immunolabeling |
| Post-embedding (on-grid) immunogold Pre-embedding immunoperoxidase or immunogold |
| Counterstaining (contrast enhancement) |
| Negative staining |
| Imaging using the transmission and scanning electron microscopes |
| Coated grids – polymer, carbon |
| Printing – large format or 35mm black and white |
| Light microscopy |
| Processing and embedding of tissue |
| Paraffin Polyester wax (low temperature embedding medium) |
| Sectioning |
| Immunohistochemistry |
Phone: 352-846-0821 Email: emcore@medicine.ufl.edu