Electron microscopy
    Preparation of fixatives
    In vivo perfusion-fixation
    Tissue dissection
    Processing and embedding of tissues or cultured cells in epoxy resins or acrylics
    Preparation of frozen tissue blocks for ultrathin cryosectioning
    Preparation of tissues for scanning electron microscopy
        Critical point drying
        HMDS drying
        Mounting
        Sputter coating
    Thick (1-micron) sectioning with toluidine blue stain
    Ultrathin sectioning – plastic embedded material or frozen samples
    Immunolabeling –
            Post-embedding (on-grid) immunogold
            Pre-embedding immunoperoxidase or immunogold
    Counterstaining (contrast enhancement)
    Negative staining
    Imaging using the transmission and scanning electron microscopes
    Morphometric analysis
    Coated grids – polymer, carbon
    Printing – large format or 35mm black and white

Light microscopy
    Processing and embedding of tissue
            Paraffin
            Polyester wax (low temperature embedding medium)
            OCT for frozen sections
    Immunohistochemistry

Phone: 352-846-0821             Email: emcore@medicine.ufl.edu