Cover Image in the JASN Paper

JASN march cover

The staff of the UF CoM EM Core contributed to the research of Dr. Susan Wall and her group at Emory University. It was recently published and was the subject of an editorial comment in the prestigious Journal of the American Society of Nephrology. The quality of the immunohistochemistry and morphologic studies was noted by the reviewers and examples of the excellent double-immunolabeling of kidney sections, done by our own Chao Chen, were selected for the cover and masthead illustrations.

The technical elements that we consider essential to producing this work are: in vivo perfusion-fixation with 3% paraformaldehyde-lysine-periodate; embedding in low melting temperature polyester wax; 2 micron-thick sections; special blocking steps for clean mouse antibody-on-mouse tissue labeling; double labeling using polymer-linked peroxidase-conjugated secondary antibodies and peroxidase substrates that produce reaction products distinguishable by color.